Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2013
  • Volume: 6
  • Issue: 2

Development and validation of RP-HPLC method for qunatitative analysis of Amlodipine besylate in pure and Pharmaceutical formulations

  • Author:
  • S. Chandra Dinda1, R.B. Desireddy2, P. Jitendra Kumar2,, A. Sai Chand2, P. Srinivasa Rao2, B. Ashok Kumar2
  • Total Page Count: 4
  • Page Number: 204 to 207

1School of Pharmaceutical Education and Research, Berhampur University, Berhampur, Odhisa

2Nalanda Institute of Pharmaceutical Sciences, Kantepudi (V), Guntur (A.P)

*Corresponding Author E-mail: putikam_j4@rediffmail.com

Online published on 6 May, 2013.

Abstract

A Validated simple, sensitive, specific and precise RP-HPLC method was developed for the determination of Amlodipine besylate in pure and pharmaceutical formulations. Method was carried on Zodiac C18 column (250mm×4.6mm×5μ particle size) using Methanol: Acetonitrile: 0.01M Phosphoric acid (75:23:2) as mobile phase. Detection was carried out by U.V at 236nm. The proposed method obeyed linearity in the range of 10–100μg/ml and met all specifications as per ICH guidelines. Statistical analysis revealed that this method can be used in routine quality control studies of Amlodipine besylate in pure and its formulations.

Keywords

Amlodipine besylate, C18 column, Reverse phase, Validation, Specificity