Method development and validation of Rilpivirine in bulk and Tablet doses form by RP-HPLC method

A new simple, accurate, rapid and precise isocratic High Performance Liquid Chromatographic (HPLC) method was developed and validated for the determination of Rilpivirine in bulk drug & its tablet doses form. The Method employs Waters HPLC system on C8 Column (4.6 x 250 mm, 5 μm) and flow rate of 1 ml/min with a load of 10μl. The Detection was carried out at 282 nm. mobile phase used as Acetonitrile and Phosphate buffer was used as mobile phase in the composition of 60:40, phosphate buffer adjusted the pH to 3.5, within a short runtime of 6 min. The retention times of Rilpivirine 2.75 min. The method was validated according to the regulatory guidelines with respect to specificity, precision, accuracy, linearity and robustness etc.