Dept. of Pharmacology and Pharmacognosy, Government College of Pharmacy, Karad, District,-Satara. 415124, Maharashtra, India
*Corresponding Author E-mail: atulrchopade@gmail.com
Online published on 6 May, 2013.
To evaluate the membrane stabilizing potential and inhibition of protein denaturation property of hydroalcoholic extract Phyllanthus fraternus Webster [PF].
Hydroalcoholic extract of PF was evaluated using the human red blood cells (HRBC) membrane stabilization method as HRBC membranes are similar to lysosomal membrane components. The prevention of hypotonicity-induced HRBC membrane lysis was taken as a measure of anti-inflammatory activity of PF extract. While the extract was also incubated with egg albumin and subjected to determination of absorbance and viscosity to assess the anti-inflammatory property.
The hydroalcoholic extract of PF in concentration of 25–800 μg/ml showed a concentration dependent inhibition of haemolysis of erythrocytes induced by hypotonic solution. The hydroalcoholic extract of PF also showed a concentration dependent inhibition of protein (albumin) denaturation with IC50 of 52 μg/ml.
From the study it can be postulated that the anti-inflammatory activity of Phyllanthus fraternus Webster could be due to its membrane stabilizing action and inhibition of protein denaturation.
Phyllanthus fraternus Webster, anti-inflammatory, membrane stabilizing, protein denaturation
