Simple Spectrophotometric Methods for the Determination of Zn- Sparfloxacin 1, 10-Phenanthroline Metal complex.

A simple, rapid, precise and accurate UV Spectrophotometric method was developed and validated for the estimation of Zn-Sparfloxacin1, 10-phenanthroline using Distilled methanol. Absorption maxima was determined and found 293nm from these solvent followed by linearity range determination which was found within the range of 5–60 μg/ml. From the linearity range, calibration curve is prepared in the range of 5–60 μg/ml and validated the various parameters in metal complex. All the experiments were carried out in triplicate, for each parameter relative standard deviation and % recoveries was calculated and the data were statistically interpreted and were found to be significant and within the limit. The proposed methods are further evaluated for precision, robustness studies. The results indicate the methods were precise and robust.