Development and Validation of RP-HPLC Method for Estimation of Risperidone in Tablet Dosage Form

A simple, specific, reproducible and accurate reverse-phase HPLC method was developed and validated for estimation of an antipsychotic drug, Risperidone in tablet dosage form. The separation was achieved by Thermo C₁₈ (250mm x 4.6mm, 5μm particle size) using acetonitrile and 0.2M potassium dihydrogen phosphate (80:20v/v) as an eluent, at a flow rate of 0.7mL/min. The injection volume was 20μl and the detection was carried out at wavelength 254nm. The retention time of Risperidone was 5.68min. The linearity was found to be in the concentration range of 2–10μg/ml with a correlation coefficient (r²) of 0.9982. The mean recovery was found to be in the range of 99.7–100.3%. The % RSD value was below 2 for intraday and interday precision indicated the present method was precise. Hence the proposed method can be used for routine analysis of Risperidone in tablet dosage form.