Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, 576104, Karnataka, India
*Corresponding Author E-mail:
Online published on 29 November, 2014.
Aripiprazole is an atypical neuroleptic drug used to manage conditions like schizophrenia and bipolar disorder. The aim of current work is to compare UV spectrophotometric and derivative techniques of aripiprazole to establish simple, sensitive method. In different spectrophotometric orders of aripiprazole, zero order has shown maximum absorbance at 217nm. Experimental wavelengths in other derivative orders were selected considering maximum absorbance. Wavelength difference and smoothing factor values were optimized to give improved spectral resolution and sensitivity. The method was linear and obeyed Beer's law in the range of 1.0–6.0 μg/mL. Linearity, limit of detection (LOD), limit of quantitation (LOQ) and accuracy were evaluated for each order as per ICH Guidelines. First order has shown highest correlation coefficient with lower values of LOD and LOQ whereas other derivative orders have shown higher values. So, first order derivative spectroscopy was identified as the best for the estimation of aripiprazole in bulk drug and formulation.
Aripiprazole, derivative spectroscopy, first order, second order, third order
