Spectrophotometric Estimation of Oxolamine citrate in Bulk and Pharmaceutical Dosage Form by Second Order Derivative Method

A simple and precise second order derivative method have been developed and validated for the estimation of oxolamine citrate in bulk and its tablet formulation. The standard and sample solutions of oxolamine citrate were prepared in distilled water. Oxolamine citrate was estimated at 257.2 nm for the second order derivative UVspectrophotometric method. Beer's law was obeyed in the concentration range of 1 to 14 μg/ml with coefficient of correlation value 0.9978 for second order derivative method. The method was tested and validated for various parameters according to ICH guidelines. The precision expressed as relative standard deviation, which was as 2.0487% for the above method. The proposed method was successfully applied for the determination of oxolamine citrate in pharmaceutical formulation. Results of the analysis were validated statistically and were found to be satisfactory. The proposed methods are simple, easy to apply, low-cost and require relatively inexpensive instruments.