1Assistant Professor (Senior), Industrial Biotechnology Division, School of Biosciences and Technology, VIT University, Vellore-632 014, Tamil Nadu, India
2School of Biosciences and Technology, Vellore Institute of Technology University, Vellore, Tamil Nadu, India
*Corresponding Author E-mail: csubathradevi@vit.ac.in, subaresearch@rediffmail.com
Online published on 10 February, 2015.
Nattokinase is an enzyme with strong fibrinolytic activity to prevent thrombolytic diseases. Nattokinase is a serine protease; it founds much application in medical industry widely. Nattokinase was purified from the culture supernatant of mutant strain Pseudomonas aeuroginosa CMSS. The optimum pH and temperature for nattokinase production was found to be 6 and 25°C respectively. Production medium supplemented with 1% sucrose and 1% SSP showed maximum nattokinase yield and activity.
Nattokinase, Pseudomonas aeruginosa CMSS, clot buster, optimization
