In-Vitro Antioxidant Activities of Various Extracts of Eugenia jambolana Leaves

The world today is not restricted to the use of synthetic medicine but also focusing more upon the herbal medication for curing various ailments. The present research was subjected to screen in vitro antioxidant activity of hydroalcoholic extract Eugenia jambolana leaves. Preliminary Phytochemical investigation was carried out on the successive extract Petroleum ether, Chloroform, Ethyl acetate, Ethanol and Hydroalcoholic extract of Eugenia jambolana leaves. It indicates the presence of Carbohydrates, Alkaloids, glycosides, Saponins, Tannins, Terpeoids, Phenols, Flavonoids, Protein, Sterols and steroids. The antioxidant activity was determined by in vitro methods such as Scavenging of ABTs Radical Method, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, Antilipid peroxidation Assay, Scavenging of Hydrogen Peroxide, Scavenging of Hydroxyl Radical by p-NDA Method, Nitric Oxide Radical Inhibition, Evaluation of total antioxidant capacity and Estimation of Total Phenol and Flavonol Contents. The IC₅₀ value of hydroalcoholic extract of Eugenia jambolana leaves for DPPH and hydrogen peroxide scavenging activity showed significant antioxidant activity in all antioxidant assays The results of this research work are promising thus indicating the utilisation of the leaves of Eugenia jambolana as a significant source of natural antioxidants.