The enhancement effect of permeation enhancers on Bisoprolol fumarate across animal membrane using Franz diffusion cell

The percutaneous permeation of Bisoprolol fumarate was investigated after application of transdermal patch containing combination of Eudragit RS100 and HPMC as polymers on rabbit skin. The enhancing effect of various permeation enhancers (Tween 80, propylene glycol and dimethyl sulfoxide) was evaluated at different concentrations. The in vitro skin permeation study was conducted on Franz diffusion cell using phosphate buffer pH 7.4 as receptor fluid. Flux, permeability coefficient and enhancement ratio were measured over 12 hour study and compared with a control patch. The permeation profile in the presence of PG at 30% concentration indicated the greatest flux as compared to Tween 80 and DMSO at same concentrations. The increase in flux was observed with increase in Tween 80 concentration where as a decrease was observed when DMSO and PG concentration was increased from 30% to 40%. Similarly an increased ER was obtained with 30% PG due to the property of PG to act both as an enhancer and a cosolvent. As a cosolvent it increases the solubilization and diffusion rate of drug. The study can be further conducted on human skin membrane to better understand the enhancing property of the penetration enhancers.