Development and Validation of RP-HPLC Method for Estimation of Zinc-Sparfloxacin 1,10-Phenanthroline Metal Complex.

The present research work describes the development of a new simple, precise, sensitive and validated RP-HPLC method for Zn-sparfloxacin 1,10-phenanthroline metal complex. The chromatographic conditions used for the separation was Phenomenex Luna C18 (250mm × 4.6mm, 5μm particle size) and mobile phase comprised of methanol: phosphate buffer (30:70 v/v) and pH was adjusted to 3 by o-phosphoric acid. The flow rate was 1.0 ml/min with detection at 293 nm. The retention time was found to be 4.357 min. The linearity was found to be in the range of 2–10 μg/ml for Zn-spar-phe metal complex with correlation coefficient of 0.9996. The proposed method is accurate with 98–102% recovery and precise (%RSD of repeatability, intra-day and inter-day variations were 0.240, 0.7989–1.1386 and 1.09–1.53 respectively). The limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.2658 and 0.8056 μg/ml respectively. Proposed method was validated as per ICH guidelines for precision, accuracy and linearity for estimation of Zn-spar-phe Metal complex. Results of the validation were found satisfactory.