Antioxidant, haemolytic activity of L-glutaminase producing marine actinobacteria isolated from salt pan soil of coastal Andhra Pradesh

Current work focuses on screening of various marine actinobacteria producing L-glutaminase collected from various salt pan soil in the coastal areas of Kothapattanam, Ongole, Andhra Pradesh. It also focuses on testing the antioxidant and haemolytic activity of L-glutaminase producing marine actinobacteria. Hydrolysis of glutamine to glutamate and ammonia is catalysed by L-glutaminase enzyme. It is used against cancer and also enhances flavour in a variety of fermented foods. Primary screening followed by secondary screening was done to identify the isolate showing highest L-glutaminase production. Further, different antioxidant assays like (2, 2-diphenyl-1-picrylhydrazyl) DPPH, metal chelating, reducing power were done to test the antioxidant activity of L-glutaminase producing isolate. Further the haemolytic activity of the isolate was performed. Strain BSAIP5 showed highest enzyme activity (1.77 IU/ml) as compared to the other isolates. Hence, this isolate was selected for further assays. BSAIP5 showed high antioxidant activity. For DPPH assay the scavenging activity was 70.25%, for metal chelating assay the result obtained was 1.07 and for reducing power the result obtained were 0.87.