Brine Shrimp Lethality Test of Andrographis paniculata

Brine Shrimp Lethality Test is a simple, reliable, rapid, cost effective bioassay for screening of biological activity and also to determine the cytotoxicity of sample under study. The test determines LC₅₀ values in μg/ml for the samples. Ten napulii were added into three replicates of each concentration of the plant extracts. Surviving brine shrimp larvae were counted after 24 hours and LC₅₀ was assessed. Results of five extracts of Andrographis paniculata subjected to electron beam irradiation at doses of 0, 2.5, 5, 7.5 and 10kGy showed that all samples possess significant biological activity and there was no cytotoxicity. This clearly indicates that, the doses at which the samples were irradiated are themselves not causing any further toxicity to the samples and thus is safe.