HPLC Method for Analysis of Bioactive Compound from Momordica charantia

Momordica charantia commonly known as bitter melon/gourd, a member of Cucarbitaceae. Qualitative phytochemical analysis of Momordica charantia confirms the presence of photochemicals like flavonoids, saponins, terpenoids, coumarins, emodins, alkaloids, proteins, etc. HPLC is a simple, accurate and selective technique for separation and quantification of anthraquinone and flavonoid and phenols from leaf sample of bitter gourd. The most common method for chromatographic determination of anthraquinone is the HPLC method. Anthraquinones are a class of natural products encompassing several hundreds of compounds, differing in the nature and positions of substituent groups. Many anthraquinones have potential therapeutic value, since antimicrobial, insecticidal, antitumor, anticongestive, hypotensive and sedative properties have been assigned to these compounds.