Optimization of Cellulase Producing Aspergillus flavus SB4 by Solid State Fermentation using Response Surface Methodology (RSM)-CCD

Solid State fermentation is widely applied to produce many enzymes and metabolites mainly from fungi. Multiple substrate (Paddy straw, Sugarcane bagasse, Rice husk and wheat bran) were mixed in different combination. Aspergillus flavus isolated from rhizosphere was inoculated. After incubation, the cellulase concentration was quantified using FPase. The optimum parameter for the production of cellulase was analysed using RSM –CCD. The results were evaluated on 28^th day of incubation. R² value was 0.989, adjusted R² was 0.9788 and predicted R² is 0.9505. The quadratic model gave a satisfactory description of the experimental data. Under optimum conditions, a maximum cellulase concentration of 55.43 IU/g was predicted according to the developed model.