Study of different parameters on biodecolourization of Direct orange (WS) 102 dye by Pseudomonas sp. isolated from soil sample

The aim of the present study was to evaluate the decolourizing and degradation activity of isolated Pseudomonas sp. on Direct Orange 102 dye. The said bacterial spp. was isolated from soil of dumping area of Nagpur (MH) India. Bacterial decolourization and screening of decolorizing bacteria were done by using dye. Colorless colonies was obtained and then it's characterization including morphological characteristics, biochemical characterization was carried out and from biochemical analysis bacteria found to be Pseudomonas sp. In further study, effect of physiochemical parameter such as temperature, pH, concentration, carbon source, nitrogen source and potassium source on decolourization of Direct Orange 102 dye at wavelength 480 nm was carried out. The analysis on dye degraded metabolite was done with TLC show difference in the Rf value confirms the degradation of dye. The isolated bacteria were co-immobilized in Ca-alginate beads and its decolourization efficiency was determined and found to be increase with increase in time.