Biosynthesis of Green Nanoparticles from Occimum sanctum and their Characterization

Development of green nanotechnology is generating interest of researchers toward eco-friendly biosynthesis of nanoparticles. In this present study silver nanoparticles were synthesized from silver nitrate (1mM) through a simple and eco-friendly route using leaf broth of Occimum sanctum as reductant and stabilizer. The silver nanoparticles was synthesized by biological method by exposing the silver ions to leaf broth which were reduced and resulted in the green synthesis of silver nanoparticles ranging from 20nm-30 nm. The bio reduced silver nanoparticles were characterized by UV-Vis spectrophotometer, X-ray diffraction, FESEM with EDAX and Fourier transform infra-red (FTIR) spectroscopy. The synthesised green nanoparticles were subjected to various analytical techniques. The UV absorption range was around 420 nm and the FTIR measurement was carried out to identify the possible biomolecules responsible for efficient stabilization of silver nanoparticles. FESEM determine the shape and size of the nanoparticle which was around 20–30 nm. XRD characterized the structure of crystalline material which showed four different distinct diffraction peaks. Thus, this method can be used for rapid and eco-friendly biosynthesis of stable silver nanoparticles of size range 20–30 nm.