Simultaneous Spectrophotometric Estimation of Atenolol and Losartan in Tablet Dosage Form

The accurate, precise, sensitive and economical Spectrophotometric method was developed and validated for simultaneous estimation of Atenolol and Losartan in tablet dosage form. The UV method employed was Simultaneous Equation Method. The method employs 274 nm as λ1 and 250 nm as λ2 for formation of equations. Atenolol and Losartan obeys Beer's law in the concentration range 10–160 μg/mL⁻¹ (r2=0.9959) and 10–160 μg/mL⁻¹ (r²=0.9979). The mean recovery for Atenolol and Losartan were found to be 100.1±0.3578% and 99.99±0.1852% respectively. The developed method were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. Thus the proposed methods were successfully applied for simultaneous determination of Atenolol and Losartan in routine industrial work.