1Research Scholar, M Pharmacy, Pravara Rural College of Pharmacy, Pravaranagar
2Assistant Professor, Pravara Rural College of Pharmacy, Pravaranagar
*Corresponding Author E-mail: kushal.landge555@gmail.com
Online published on 16 September, 2020.
Ramipril is a potent long acting ACE inhibitor and Hydrochlorothiazide is a thiazide diuretic and an antihypertensive. In literature few analytical methods are discussed for the estimation of Ramipril and Hydrochlorothiazide. The objective of this study is to develop and validate RP-HPLC method for the qualitative estimation of the drug. In most of the methods, the isocratic elution was carried out by using Acetonitrile, water and buffer as a mobile phase. The wavelength was in between 210 to 215nm for Ramipril and 316nm for Hydrochlorothiazide. The flow rate was approximately 0.8ml/min to 1.0ml/min. The temperature set was ambient temperature. The developed methods were validated as per ICH guidelines and this method were found to be specific, precise, sensitive and robustness.
Ramipril, Hydrochlorothiazide, RP-HPLC method, Antihypertensive, ACE inhibitor, Validation. System suitability, Stability-indicating, Pharmaceutical dosage form, ICH
