1Department of Pharmaceutics, Institute of Pharmacy, Loni, India
2Department of Pharmaceutical Chemistry, Institute of Pharmacy, Loni, India
*Corresponding Author E-mail: shinde.ganesh@pravara.in
Online Published on 06 May, 2024.
To develop a simple, sensitive, accurate, precise and rapid UV spectrophotometric method for the determination of metformin in human urine.
Extraction of metformin from urine samples was done by Dilute and shoot method in which urine was diluted upto 1:10 ratio to avoid interference of matrix. For estimation of Sitagliptin the solvent system employed was water. The sitagliptin was scanned in range of 200nm to 400nm and wavelength of detection (λdet) was found to be 234nm.
The calibration curve was linear in the range of 1-17μg/ml. The recovery and assay studies of sitagliptin were within 93-94.85% indicating that the proposed method can be estimation of sitagliptin.
Sitagliptin, Urine, Bioanalytical Method, UV Spectrophotometer
