1Guru Nanak Institute of Pharmaceutical Science and Technology, Panihati, Kolkata, West Bengal
2Associate Professor, Guru Nanak Institute of Pharmaceutical Science and Technology, Panihati, Kolkata, West Bengal
**Corresponding Author E-mail: prerona.saha@gnipst.ac.in
Online published on 21 May, 2025.
Borassus flabellifer L. known as Tal in West Bengal belongs to the Arecaceae family. In the present experiment, we used the endosperm part (germinated sprout). The edible sprout part is a familiar food of the people. However, its flavonoid profiling is not yet reported.
This study aimed to explore the presence of quercetin and its quantitative analysis in the endosperm of Borassus flabellifer.
TLC was carried out with an established solvent system toluene: ethyl acetate: formic acid (5:4:0.2, v/v/v), to detect quercetin. A validated HPTLC method was devolved by using silica gel plates with the established solvent system used in TLC.HPTLC analysis was carried out with the same solvent system toluene: ethyl acetate: formic acid (5:4:0.2, v/v/v). Quantitative estimation was done from the AUC obtained in the plot.
The method was confirmed to be specific, and accurate across different measurements. Recovery studies showed recovery 108.50 with RSD (relative standard deviation) % 0.84 of quercetin is retrieved accurately. The germinated sprout was found 0.98mg/100g of quercetin.
The HPTLC is an efficient and sensitive tool for the analysis of quercetin in the sample extracts in available products. This is the first-ever described method for this specific purpose, potentially paving the way for quality control and standardization of herbal products of B. flabellifer
Quercetin, Borassus flabellifer, HPTLC, Method Validation, Bioactive
