Research Journal of Topical and Cosmetic Sciences

Acne, common skin disorder in which follicles becomes blocked with sebum, dead skin cells and bacteria that causing skin redness and inflammation. Extract of plant parts were extracted by hot soxhlet extraction process using hydroalcoholic sovent (50:50). Dried extracts of Azadirachta indica leaves and Nigella sativa Seeds, Aloe barbadensis leaves pulp and essential oil of Melaleuca alternifolia were incorporated into gel base contains polymer carbopol (Grade 934 or 940) and formulated into gel. The gel was evaluated significant antibacterial property of Polyherbal gel formulation using Muller-Hinton agar plates against Staphylococcus aureus and Staphylococcus epidermidis while Reinforced Clostridial agar plate was prepared for evaluating antimicrobial activity against bacteria Propionibacterium acnes. Different physicochemical properties include color, appearance, consistency, washability, pH, spreadability, extrudability, viscosity, homogeneity etc. The prepared formulations did not show any phase separation it was stable and gel did not showed any irritation over skin. The result concluded that the developed 10% Polyherbal gel formulations (AgF1 to AgF10) are appropriate formulations and all formulations showed significant zone of inhibition against acne causing bacteria’s.