Range Management and Agroforestry

Open Access
SCOPUSWeb of Science
  • Year: 2010
  • Volume: 31
  • Issue: 2

Molecular characterization of two cultivars of Egyptian clover (Trifolium alexandrinum L.)

  • Author:
  • E. M. Zayed1,, Magda I. Soliman2, G. A. Ramadan1, M. M. Tarrad1
  • Total Page Count: 4
  • DOI:
  • Page Number: 140 to 143

1Forage Crops Research Department, Field Crops Research Institute, Agriculture Research Center, Giza-12619, Egypt.

2Department of Botany, Faculty of Science, Mansoura University, Mansoura, Egypt.

* Corresponding author e-mail: ehabzayed31@hotmail.com

Online published on 11 January, 2012.

Abstract

Two Egyptian clover cultivars namely ‘Fahl’ and ‘Helaly’ were analyzed based on seed soluble protein pattern as well as RAPD, ISSR and AFLP generated DNA profiles. For AFLP analysis, DNA of Egyptian clover cultivars were digested with EcoRI + MseI restriction enzymes. Among the molecular markers used ISSR showed highest level of molecular variance (24.5%). In terms of allele frequency (p), level of difference between two cultivars was variable. Results also indicated that relationship study between two cultivars can be better performed using Cubic, Quadratic model.

Keywords

AFLP, Egyptian clover, Fodder, ISSR, Molecular markers, RAPD, Trifolium