SKUAST Journal of Research
Open Access
  • Year: 2012
  • Volume: 14
  • Issue: 1and2

Comparative Efficacy of Rectal Pinch, Faecal Smear and Faecal Polymerase Chain Reaction Tests for Surveillance of Paratuberculosis in Goats (Capra hircus)

  • Author:
  • Imran Hamid Shah, Mohammed Maqbool Darzi, Masood Saleem Mir
  • Total Page Count: 7
  • Page Number: 17 to 23

Division of Veterinary Pathology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Shuhama – 190 006, Ganderbal, Jammu & Kashmir (India)

*Email: masoodmir1@gmail.com; masoodmir1@skuastkashmir.ac.in

Online published on 27 January, 2014.

Abstract

The efficacy of three diagnostic tests viz., faecal smear examination (concentration method), rectal pinch examination and faecal polymerase chain reaction for IS-900 was evaluated in 100 goats. The prevalence of the disease was observed to be 34, 5 and 8% by faecal smear examination, rectal pinch examination and faecal PCR, respectively. Prevalence was 38% on combining the results of all the three diagnostic tests. Faecal smear examination, rectal pinch examination and faecal PCR were 89.5, 13.2 and 21.2% sensitive, respectively. Feacal smear examination appeared to be rapid, cheap and reliable test for screening both clinical and sub-clinical cases of paratuberculosis under field conditions.

Keywords

Diagnostic tests, efficacy, faecal smear, paratuberculosis, PCR, rectal pinch