SKUAST Journal of Research
Open Access
  • Year: 2016
  • Volume: 18
  • Issue: 2

Stock Identification of Nemipterus japonicus Along West Coast of India Using RAPD Markers

  • Author:
  • H. Mudasir1,, M.S. Sawant2, R.A. Pawar2, A.S. Pawsae2, F.A. Bhat3
  • Total Page Count: 8
  • Page Number: 130 to 137

1Division of Animal Biotechnology, FVSc & AH, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar – 190 006, Jammu and Kashmir (India)

2College of Fisheries, Rantnagiri, DBSKKV-415 712

3Faculty of Fisheries, Rangil, SKUAST-K-190006

*E-mail: hmudasirm@gmail.com

Online published on 25 November, 2016.

Abstract

Genetic structure of six populations of Nemipterus japonicus from Veraval (Gujarat), Ratnagiri (Maharashtra), Mumbai (Maharashtra), Panaji (Goa), Malpae (Karnataka) and Kochin (Kerala) in India was studied using randomly amplified polymorphic DNA (RAPD) markers. Two selective primers provided distinct and consistent results. The RAPD profiles generated by both primers revealed varying degrees of polymorphism, ranging from 57.92 (primer OPA-01) to 69.01 (primer OPA-09). Nei's genetic diversity (h) among the four populations varied from 0.2680 ± 0.2396 (Veraval population) to 0.4406 ± 0.0537 (Malpe population). Nei's highest genetic distance (0.3168) was observed between Veraval and Panaji populations.

Keywords

Genetic structure, Nemipterus japonicus, randomly amplified polymorphic DNA (RAPD), stock structure, west coast India