SKUAST Journal of Research

Open Access
  • Year: 2019
  • Volume: 21
  • Issue: 1

Standardization of sterilization protocol of explants for micropropagation in tomato (Solanum lycopersicum) male sterile line Shalimar FMS-1

  • Author:
  • Syed Mazahir Hussain1,, K. Hussain1, Ajaz A. Malik1, Amjad Husaini2, S.A. Mir3, K.M. Bhat4, Majid Rashid1, Syeda Farwah1, Seerat Rizvi1, Sameena Maqbool1
  • Total Page Count: 7
  • DOI:
  • Page Number: 79 to 85

1Division of Vegetable Science, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar-190 025, Jammu and Kashmir (India)

2Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar-190 025, Jammu and Kashmir (India)

3Division of Agri-Statistics, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar-190 025, Jammu and Kashmir (India)

4Division of Fruit Science, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar-190 025, Jammu and Kashmir (India)

Abstract

Surface sterilization is a vital step in preparation of explants for tissue culture as plants in the field are highly exposed to microbial contamination An improved method for sterilization of explants of tomato male sterile line Shalimar FMS-1 was standardized for in vitro micropropagation. Among twenty one sterilization treatments sodium hypochlorite @ 1% for 45 seconds proved to be most efficient as it resulted in highest survival, minimum mortality and minimum contamination of explants.

Keywords

Explants, survival, sterilization, tomato