1Division of Animal Biotechnology, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir
2Division of Veterinary Microbiology and Immunology, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir
3Division of Veterinary Pathology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir
4Department of Biotechnology, University of Kashmir
*e-mail: nadeem.shabir@skuastkashmir.ac.in
Online published on 24 May, 2021.
Infectious bursal disease (IBD) virus is a double-stranded (ds) RNA virus belonging to genus Avibirnavirus and family, Birnaviridae. The virus causes infectious bursal disease, an immunosuppressive viral disease of poultry possessing huge economic impact on poultry industries globally. Two infectious bursal disease viruses were isolated and cultured in chicken embryo fibroblasts, derived from 9-day old chicken embryo. The cytopathic effects typical to that of infectious bursal disease virus were observed after adaptation of the virus in chicken embryo fibroblasts following three blind passages in cells. The virus was detected using a one-step RT-PCR and validated using one step real-time PCR. A IBD virus quantification procedure was standardized to evaluate the infectious copy number of the virus. The virus was further passaged and tittered using serial dilution method following which it was stored in -80°C till further use. The isolation of an IBD virus from vaccinated chicken indicates either a reversion-to-virulence event of live-attenuated IBD vaccine or insufficient protection offered by the currently used live attenuated IBD vaccines for which further investigation is required.
IBD virus, Virus amplification, Virus isolation, Virus quantification
