1Division of Floriculture and Landscape Architecture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir-190025, India
2Division of Basic Sciences and Humanities, SKUAST-K, Shalimar
3Division of Vegetable Science, SKUAST- K, Shalimar
4Division of Soil Science, SKUAST-K, Shalimar
*e-mail: wanitabinda09@gmail.comm
Online published on 30 September, 2025.
Lisianthus (Eustoma grandiflorum) is a globally popular cut flower valued for its rose-like appearance, vibrant colour range, and long vase life, making it a favourite in the floral trade and ornamental gardening. aimed to optimise sterilisation protocols for Lisianthus nodal explants using mercuric chloride and sodium hypochlorite, testing their various concentrations and exposure times forenhancement of in vitro propagation success. The results showed that mercuric chlorideat 0.05% improved asepsis with extended exposure but reduced explant survival. Meanwhile, sodium hypochlorite 4% with extended exposure reported maximum explant asepsis of71.67% and commendable explant survival (80.33%).
Asepsis, Lisianthus, Nodal explant, Sterilisation, Survival
