Amino Acid Variability in Coat Protein Gene of Mungbean Yellow Mosaic India Virus Infecting Pulse Crops

The coat protein gene (CP) of Mungbean yellow mosaic India virus (MYMIV) was successfully amplified from four pulse crops (mungbean, urdbean, cowpea and rajmash) showing yellow mosaic symptoms using specific primer pair AV1-F- 5’ GTA TTT GCA (GT)CA(AT)GTTCA3’/AV1-R- 5’AGG DGTCAT TAG CTT AGC 3’. The complete nucleotide sequence of the coat protein gene of all the MYMIV isolates had single open reading frame (ORF) of 774 bp and 257 amino acids. Comparision of amino acid sequences of CP genes revealed that isolates understudy had 97–99% sequence homology with known isolate of MYMIV-Cowpea (AF481865). Variability in amino acids sequences in the MYMIV isolates understudy with the type isolate has been observed at 6 positions only. This indicates that the CP gene of MYMIV is highly conserved though the gene has been isolated from different pulse crops.