Genotypic Identification of Lentil (Lens culinaris) using Electrophoresis Technique

Lentil (Lens culinaris) genotypes were selected for identification, namely Asha, DPL 15, DPL 62, IPL 81, JL 3, K 75, L 4076, LL 147, LL 4147, LL 56, LL 699, PL 234, PL 406, PL 5, Ranjan and Subrita using SDS PAGE and PAGE using electrophoresis techniques. Lentil genotypes were distinguished based on presence and absence of protein bands at perticular Rm value. SDS PAGE and PAGE gave the good banding pattern, however, the banding pattern through SDS-PAGE gave more polymorphism for characterization of lentil genotypes based on soluble seed protein. Similarly esterase isozymes at high concentration (12% running gel) showed good polymorphism. Electo phoretic can be utilized efficiently for genotypic identification.