Determination of antimycobacterial activity of polyenzyme preparation Immunoseb-S using In vitro methods

The polyenzyme formulations developed by Advanced Enzyme Technologies Ltd., Thane, Exclzyme and Immunoseb-S, were tested for their ability to inhibit the growth of the tubercle bacilli. For this, the liquid medium method (REMA) was used. It was found that both the formulations have antitubercular properties and can inhibit the growth of M tuberculosis at a Minimum Inhibitory Concentration of 5 and 10 mg/ml respectively. Although it was not possible to show any potentiation of standard drugs by in vitro methods, a detailed study using MDR strains and sensitive strains showed that the novel formulations are equally effective against both sensitive and MDR strains. Exclzyme and Immunoseb-S may hence be a major breakthrough in the management of MDR tuberculosis.