Trends in Biosciences

  • Year: 2012
  • Volume: 5
  • Issue: 3

High Frequency Plant Regeneration Through In Vitro Cormel Formation Using In Vitro Root as Explant of Gladiolus sp.

  • Author:
  • Arvind Arya1,, Sandeep Kumar2
  • Total Page Count: 3
  • DOI:
  • Page Number: 168 to 170

1Department of Biotechnology, Meerut Institute of Engineering and Technology, Meerut. U.P.

2National Institute of Engineering and Technology, NIMS University, Jaipur

Abstract

The direct organogenesis was derived from roots of in vitro plantlets as an explant in Gladiolus sp. (Iridaceae), a highly valuable ornamental variety. Direct shoot buds were initiated at a higher frequency from in vitro roots of Gladiolus sp. used as explant. Different basal medium viz., MS, B5 and 2iP were tested and found MS medium best for initiation of shoots. Highest number of shoots regenerated in 1x MS with 2.0 mg/l BAP. The shoots produced roots in MS with 1.0 mg/l IBA and 6% sucrose. After five weeks, rooted plantlets were transferred for initiation of cormels. The protocol enabled to harvest more than 50,000 cormels (cormlets) within 150 days starting from a single in vitro root explant.

Keywords

Cormel, Gladiolus, Ornamental, Regeneration, Root explant