Trends in Biosciences
  • Year: 2012
  • Volume: 5
  • Issue: 3

Establishing Callus Formation of Swarna and IR 64 and Sub-culturing of Callus and Monitoring the Stages of Regeneration

  • Author:
  • Kshitij Kumar, K.N Singh
  • Total Page Count: 5
  • Page Number: 171 to 175

Department of Plant Molecular Biology & Genetic Engineering, Narendra Deva University of Agriculture & Technology Narendra Nagar (Kumarganj), Faizabad (U.P)

Online published on 18 February, 2013.

Abstract

The present regeneration from the target tissue (explant) of the genotype (s) is concerned. The present study was conducted with an objective to standardize efficient regeneration system for IR64 and Swarna at N.D.U.A.T (U.P.) India. MS medium supplemented with 2,4-D, (2mg/l) kinetin, (0.4mg/l). L. Proline, (500mg/L) Casein Hydrolysate, (300mg/L) agar-agar (0.8%w/v) was found best callus medium for Swarna. MS medium supplemented with 2,4-D, (2mg/L) L-Proline (500mg/L) maltose (30g/L) agaragar (0.8%w/v) was found to be best callus medium for IR64.

Keywords

indica rice, IR64, regeneration, rice, 2, 4-D, Swarna