Trends in Biosciences

  • Year: 2013
  • Volume: 6
  • Issue: 1

Expression and Immunogenicity of E2 Glycoprotein Gene of Classical Swine Fever Virus Cloned in Eukaryotic Expression Vector

  • Author:
  • Nitin Sharma1,, Purshotam Kaushik1, Anant Rai2
  • Total Page Count: 5
  • DOI:
  • Page Number: 23 to 27

1Department of Botany & Microbiology, Gurukul Kangri Vishwavidyalaya, Haridwar-249 404, UK, India

2Institute of Biotechnology & Information Technology, 197, Mudia Ahamadnagar, Pilibhit Road, Bareilly-243 122, Uttar Pradesh, India

Abstract

The E2 glycoprotein of classical swine fever virus (CSFV) is immunogenic and induces neutralizing antibodies against CSFV. Inthe present studythe recombinant plasmid pSin.csfv.E2 transfected HeLa cell expressed E2 protein which was confirmed by immunoperoxidase test. The in vivo immunological response was studied in pigs immunized through IM route. The immune response on 28th day post vaccination induced by 10 μg dose of the rplasmid revealed protective serum neutralizing antibody titer 60, while control group showed lower 1:5 non protective SN titer. Stimulation Index (SI) was found higher in vaccinated group as compared to healthy control animals. The T-cell response in the blood of vaccinated pigs suggested the role of cell mediated immunity in protection apart from neutralizing antibodies in serum. The challenge test revealed 100% protection of vaccinated pigs while all control pigs died of csfv infection. Therefore, this study demonstrates that DNA vaccination with 10 ig dose of pSin.csfv.E2 can provide protective immunity 28 days post vaccination.

Keywords

Classical Swine Fever Virus, DNA vaccine, E2 gene, immunity