1Department of Biotechnology, Indian Institute of Horticultural Research, Bengaluru, 560 089, Karnataka, India
2Department of Plant Science, University of Hyderabad, Hyderabad, 500 046, Andhra Pradesh, India
3Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bengaluru-560 065, Karnataka, India
*E-mail: mrgyadav@gmail.com
Online published on 23 May, 2013.
Peanut bud necrosis virus (PBNV), a tospovirus has become serious disease in major tomato (Lycopersicon esculentum) growing areas of the world. The virus was obtained in purest form from naturally infected tomato sample. The virus was detected by DAC-ELISA using polyclonal antibodies against PBNV and TSV. PBNV was succesfully transmitted to Vigna unguiculata, Nicotiana rustica and Nicotiana benthamiana.
Lycopersicon esculentum, PBNV, tospovirus, TSV, ELISA
