Efficient Protocol for In Vitro Regeneration in Pigeonpea (Cajanus cajan (L.) Millsp.)

The present study precisely emphasized on the establishment of a reproducible regeneration protocol through different explants in pigeonpea [Cajanus cajan (L.) Millsp.] using different explants like primary leaf, epicotyls, cotyledonary node and embryonic axis in variety ‘GT 100’. In vitro raised fourteen days old seedlings grown aseptically on Murashige and Skoog's medium were most efficient in producing multiple adventitious shoot from cotyledonary node explants and shoot bud originated from the petiolar cut end of explants. Efficient regeneration in pigeonpea from callus was obtained using MS medium supplemented with growth regulator viz; 5 ìM Gibberellins for shoot elongation, precise combination of BAP (22 ìM) and kinetin (10 ìM) for formation of shoot buds and 10ìM NAA for root initiation under precise conditions.