Studies on Antimicrobial Compounds of Extract of Bark of Sonneratia alba

This study was aimed at evaluating antibacterial potential of the Sonneratia alba in attempt to identify potential natural sources for synthesis of new drug to avoid the growing antibacterial resistance. The peals of bark were extracted by cold and hot methanolic extract by Alade and Irobi's and Soxhlet extraction method. Antibacterial activity of crude extract showed promising activity against tested organisms. The MIC of crude hot methanolic extracts was >2.5 mg/ml and cold methanolic extract were 1.25 mg/ml. The methanolic crude extract was subjected for HPTLC analysis shows 11 peaks indicates approx. 11 different phyto-compounds such as glycosides, tannins, saponins and alkaloids. The hot methanolic crude extract was further subjected to activity guided fractionation with different polarity solvents, showed varying levels of bactericidal activity. Fraction E[acetone] and fraction F[methanol] shows maximum activity than other fractions. HPTLC and bioautography was performed for both fractions and phytochemical analysis shows mainly presence of flavonoids and saponin. Preparative HPTLC was performed to obtained semi purified bioactive compound and separated band were subjected to AST, UV spectroscopy, GCMS, FTIR, CHNS(O) analysis and NMR spectroscopy. By comparing with GCMS library matches most of these probabilities and may be the compound of interest viz; N-â-Chloropropinoyltryptamine in fraction E and Myo-Inositol, 4C methyl in fraction F. Both of the compounds were confirmed by performing NMR spectroscopy and matching its dell values.