Genetic Diversity of G6PD Gene and ABO Blood Groups Among the Four Communities of Madhya Pradesh, India

Glucose 6-Phosphatase Dehydrogenase (G6PD) deficiency is probably the most common enzymopathy in human. Analyzing this gene system affords an opportunity relate the naturally occurring genetic variation in the human species (microevolution) to the macroevolution. The current study was aimed to find the diversity of exon3 of G6PD gene sequence among the four communities residing in Madhya Pradesh, India. 144 subjects (66 male and 77 females) were studied from four different communities as, 32 Gond (Bheempur, Chiholi), 42 Bansod (Betul), 37 Kunbi (Tembhurni, Athner) and 33 Baiga (Khajuri, Mandla). SNP-BLAST analysis of 144 exon 3 sequences revealed no previously described critical (mutation) variation. The BLASTX analysis identified the presence of CDS from average ∼ 127 positions. The Multiple Sequence alignment and phylogenetic analysis has revealed the maximum variation among the sequences of Baiga subject, followed by Gond. High level of similarity was observed among the sequences of subjects belonging to Kunbi and Bansod. Overall B blood group was most prevalent beside blood group AB and blood group A was least prevalent among the four populations of communities.