Comparative Study of different DNA Extraction methods for molecular detection of Late Blight of Potato, caused by Phytophthora infestans

The Polymerase Chain Reaction (PCR) has greatly simplified procedures for cloning and modification of nucleic acids. PCR is also used for efficient detection of specific DNA sequences in individuals, but when large populations are to be assesses, it is important for the procedure to be simple and reliable. a comparison was made with the all three different extraction methods discussed and it was found that glass powder method is more reliable than the Readymade kit and CTAB method. The aimof the present study was to develop a simple, rapid and inexpensive protocol of fungal genomic DNA extraction suitable for use in detection of P.infestans. This procedure enables large numbers of samples to be easily prepared for PCR analysis per day. The importance of this study was to standardize and develop a simple reliable protocol for isolation of PCR – ready fungal genomic DNA.