Improved and Convenient Method of High Quality DNA Isolation from Grasspea (Lathyrus sativus L.): A Protein Rich Crop

DNA extraction from the pulses crop like grasspea is often problematic due to presence of proteins and different metabolites. In order to overcome the problems associated with poor-quality DNA yield from such plant samples, a protocol has been developed, whichinvolves inactivation of contaminant proteins by using Protinase-K and precipitation of polysaccharides in the presence of high salt concentration. The purity of genomic DNAwas confirmed by A_260/280 ratios calculated from the spectrometric readings and further RAPD analysis of isolated DNA. Isolation of sufficiently higher amount of DNA from leaf samples of grasspea is feasible from this protocol. In this study, twenty grasspea genotypes have been included for standardization of this protocol and further validation of extracted DNA for PCR amplification and other molecular works have also been considered. Thus, the new protocol for genomic DNA extraction can be successfully used for protein rich crops like grasspea.