Determination of Lethal Dose and Biological Injury by Gamma Rays and Ethyl Methane Sulphonate in M₁ Generation of Blackgram (Vigna mungo (L) Heppper)

This study was carried out to determine the lethal dose/concentration of gamma rays and EMS in two promising blackgram varieties viz., MDU (Bg) 1 and VBN (Bg) 4. The lethal dose was determined by probit analysis. The impact of mutagen on biological parameters also studied. The LD₅₀ was fixed at more or less equivalent dose/concentration of both mutagens and varieties. The biological parameters such as seed germination, shoot length, root length, plant survival on 30 days after sowing, plant height on 30 days after sowing, pollen fertility, plant height at maturity and seed fertility showed decreasing pattern with increase of mutagen dose/concentration. The maximum reduction was noticed for the traits viz., seed germination, shoot length, root length, plant survival and pollen fertility.