Estimation of Seed Storage Protein and Molecular Weight Determination in Micro Mutant Lines of Green Gram (Vigna radiate (L.) Wilczek) by SDS-PAGE

Seed protein analysis (albumin and globulin) through SDS-PAGE was done to assess the status of genotypes for protein quality and molecular characterization of 30 greengram genotypes. Electrophoresis results revealed 12 albumin and eight globulin polypeptide bonds with different molecular weights. The polypeptide band AL3 was medium thick but sharp in all genotypes except genotype SN3-3, SG3-3, SE2-3, SM1-3, SE2-2, SG1-2, OGN2-3 and OGM2-3 indicating absence of gene responsible for production of that polypeptide in them which can be used as marker for those particular genotype. Similarly GL1 and GL3 which were faint and thin in nature were absent in few genotypes and can be used as molecular marker for genotypes OG3-2, SN1-2 and SE3-2 respectively for their identification.