Purification of Mycelial Proteins of Colletotrichum gloeosporioides and Lasiodiplodia theobromae of Mango for Production of Polyclonal Antibodies

Anthracnose, caused by Colletotrichum gloeosporioides and stem end rot caused by Lasiodiplodia theobromae are the major diseases of mango, limiting fruit production. Nineteen isolates of C. gloeosporioides and sixteen isolates of L. theobromae were collected from the major mango growing regions of Tamil Nadu, India. The crude mycelial proteins of the isolates of the two pathogens were extracted and the variation in the protein profile within the isolates was determined. The common protein expressed by the isolates was identified, purified and used as antigen for raising antibodies.