1Lokmangal College of Agricultural Biotechnology, Wadala, Maharashtra, India
2Faculty of Agriculture, Ehime University, Japan
3Tamil Nadu Agricultural University, Coimbatore, India
4Korea Advanced Institute of Science and Technology, Korea
5UAS, GKVK, Bangalore, Karnataka, India
6Jawaharlal Nehru Krishi Vishva Vidyalaya, Jabalpur, Madhya Pradesh, India
7PhD Scholar, TUAT, Tokyo, Japan
*Email: saffy.shahakar@gmail.com
Online published on 7 January, 2015.
A successful in vitro culture establishment has been achieved from the leaves of blue passion flower (Passiflora caerulea L) and the regeneration of shoots was observed with and without callus development. Leaf segment of Passiflora caerulea L. formed callus when cultured on modified Murashige and Skoog (MS) medium supplemented with 0.5 mg/l NAA, 0.5 mg/l IAA, 0.5 mg/l 2-4-D, 0.5 mg/l Kinetin and 1 mg/l BAP. Direct shoot regeneration without a callus phase has also been achieved from the leaf segment in presence of 2 mg/l BAP and 1 mg/l kinetin. According to our study, a complete plant of blue passion flower along with shoots and roots was developed in around 54 days, which may take near about two seasons or sometimes a year for a seed of blue passion flower to germinate.
Micro propagation, Passiflora caerulea L., leaf segment, NAA, BAP
