Isolation and Identification of a Novel, Cold Active Lipase Producing Psychrophilic Bacterium Pseudomonas vancouverensis

Psychrophiles are particularly important due to flexible structure of their enzyme and tremendous potential for use in low temperature processes. A potential lipase producing novel psychrophilic bacteria G3 was isolated from rice rhizosphere of Nainital, India. The microscopic study showed it was gram negative, aerobic, rod shaped and motile organism. It wasable to grow at 4ºC to 37ºC and at wide range of pH 5.0 to 10.0. On solid media colonies were round, smooth, viscous and light yellow in colour. 16s rDNA gene sequencing and bacterial identification was done. The obtained nucleotide sequence (1388 bp) compared with the NCBI databases through BLAST. The sequence showed 100% similarity with Pseudomonas vancouverensis-A-18 (HQ202824.1) and lowest e-value, so based on the identity unknown bacterium was identified as Pseudomonas vancouverensis. It was able to produce maximum extracellular lipase at its optimum growth conditions i.e. 48 h incubation period at 10°C and 8.0 pH.