Deptt. of Microbiology, CCS Haryana Agricultural University, Hisar
Online published on 13 January, 2016.
Clusterbean (Cyamopsis tetragonoloba (L.) Taub) is a kharif legume crop grown under arid zone in India. Root rot is the major disease in clusterbean caused by Rhizoctonia solani during rainy season and may result upto 21.60% loss at pre-and post-emergence stages. Forty two isolates of r hizobacteria were obtained from soil samples collected from rhizosphere of clusterbean and thirteen bacterial isolates were obtained from dilution plating of farmyard manureobtained from clusterbean grown field. All the 55 bacterial isolates were screened for antagonistic interactions against Rhizoctonia solani on PDA medium plates. Pseudomonas/Bacillus isolates HCS2, HCS4, HCS30, HCS36, HCS43 and HFS12 showed significant antagonistic activity. Eleven rhizobactrial isolates showed stimulation of root and shoot growth of clusterbean on water agar plates and Isolates HCS2, HCS4, HCS12, HCS13, HCS30, HCS36, HCS39, HCS43, HFS8, HFS10 and HFS12 showed retadation as compared to uninoculated seedlings. Rhizobium isolate GSA110 formed 45 nodules and nodule weight observed was 388.3 mg/plant, and 228.19% increase in shoot dry weight was observed as compared to uninoculated control at 60 days of plant growth. Coinoculation ofBradyrhizobium GSA11, Bacillus isolate HCS43 with fungus showed increase in nodulation (50 nodules/plant), nodule weight (317.7 mg/plant) leading to increase in shoot dry weight (209.6%) as compared to single inoculated control. Inoculation with Bacillus isolate HCS43 and Bradyrhizobium strain GSA11 showed disease suppression and browning of collar root was reduced.
Clusterbean, Antifungal, Rhizoctonia solani, Pseudomonas, Bradyrhizobium, Rhizobium, Coinoculation, Nodulation, Plant Growth
