Agrobacterium rhizogenes Transformed Hairy Root Induction from Daucus carota and Optimization of Culture Parameters for Proliferation of Transformed Roots

This study explains the conditions optimized for the production of hairy roots from the explant Daucus carota. Two MTCC strains of Agrobacterium rhizogenes 532 and 2364 were compared at different growth incubation periods. Full and half strength MS media were used to find out the best root induction per cent. The root induction was observed good in the strain 532 when half strength MS medium at an growth incubation of 48 hrs time without any addition of external phytohormones. The induced hairy roots were excised and subcultured under different media composition (MS, MSR and B5 Gamborg's medium), different gel concentrations (Phytagel 0.3,0.5% and Tissue culture agar 1%) and at different light incubations (complete and partial darkness) for the proliferation of hairy roots. The MSR medium with 0.3% phytagel under complete darkness was found to proliferate good in terms of enhancing the length of hairy roots and development of its lateral roots.