Trends in Biosciences
  • Year: 2014
  • Volume: 7
  • Issue: 9

Characterization of Sunflower Hybrids and Parental Lines by Seed Peroxidase Isozymes

  • Author:
  • Sachin B. Thawari1,, H. E. Patil, R.L. Bhakre3, R.S. Mali4
  • Total Page Count: 3
  • Page Number: 803 to 805

1Agricultural Botany Section Anand Niketan College of Agriculture, Warora (M.S.)

3Maharashtra State Seed Corporation Ltd., Jalgaon (M.S.)

4Seed Monitoring Cell, Dr. PDKV, Akola (M.S.)

*E-mail: sachinthawari@gmail.com

Online published on 25 December, 2014.

Abstract

Seed peroxidases profiling was undertaken for characterization of five sunflower hybrids and their parents. Limited qualitative and negligible quantitative polymorphism was revealed peroxidases for identification of the sunflower hybrids and their parents. CMS 21A x ID 3376 and its parents could not be discriminated with peroxidases isozymes. Hybrids, KBSH 1 and PKVSH 27; male parent in hybrids, CMS 240A x ID 5016 and CMS 336A x ID 1020; and female parent of hybrid, KBSH 1 could not be identified with peroxidases isozymes. Lack of adequate number of exclusively genotype specific isozyme markers was the major limitation for identification of the hybrids and their parents. Quantitative differences in isozyme profiles were not enough contrasting to distinguish the hybrids and their parents except PKVSH 27 and its parental lines.

Keywords

Sunflower, Peroxidase, Isozymes, Characterization