Optimization of Enzymatic Clarification of Pineapple Pulp with Multi Enzymes Using Response Surface Methodology

Pineapple is one of the major fruit crop in the world and clarified juice of pineapple is always high in demand. The study was carried out with the aim to prepare good quality clarified pineapple juice with high yield using multi enzymes system. The clarified pineapple juice was prepared by treating pulp with various level of cellulase (0.15–0.75%), xylanase (0.15–0.75%), and pectinase (0.1–0.5%) in combination at different temperature (35–55°C) for treatment period of 1 hr. The trial run for the treatment was designed using design expert (9.0.1) software and condition optimized using response surface methodology. The optimized condition achieved for clarified pineapple pulp with 83.9% clarity and 91.2% yield is treatment of pulp with 0.6% xylanase, 0.6% cellulase and 0.2% pectinase enzymes in combination at 45°C for 1 hr.