Isolation and Characterization of Yeast Isolates for Indole 3 Acetic Acid Production from Hassan District

Experiment was conducted on isolation and characterization of yeast isolates for indole 3 acetic acid production from Hassan district at Department of Agricultural Microbiology University of Agricultural Sciences, Bengaluru. Leaf and fruit samples of plantation [Coffee (Coffea robusta and Coffea arabica), Cardamom (Elettaria cardamom) and Cocoa (Theobroma cocoa)] and fruit [Sapota (Manilkara zapota), Guava (Psidium guajava), Carambola (Averrhoa carambola) and Ficus (Ficus mysorensis)] crops were collected from Hassan district. Totally 84 yeast isolates were isolated from collected leaf and fruit samples and these were screened for indole 3 acetic acid production (IAA). Among 84 yeast isolates maximum (0.387 μg/ml) IAA production was recorded by the yeast isolate TCL-1 which was identified as Meyerozyma caribbica based on the partial sequence of 18S rRNA gene from the sequence analysis and compared with other 18S rRNA gene sequences of yeasts from database (NCBI).